The principle of the protein analyzer is to detect the reaction between the antigen suspended in the buffer and the corresponding antibody to form immune complex particles under the premise of excess antibody. The light source adopts a dual-light source silicon iodide crystal bulb. When the light beam passes through, the scattering light rate of the suspended particles changes in proportion to the antigen concentration. When the reaction reaches the highest peak, the amount of product formation is measured. The level of the peak rate is related to the antigen. The content is proportional, and this measurement method is called rate nephelometry.
The instrument adjusts the background signal before the initial stage of the reaction to a rate value equal to 0, and then as the antigen-antibody binding reaction proceeds, the enhancement of the rate signal gradually accelerates, and finally reaches the peak. Once the required rate peak is measured, the instrument will also verify the peak through the peak identification program to eliminate the disturbing scattered light signal caused by contaminated particles, bubbles and non-specific precipitates.
The protein analyzer is composed of four parts: a host, a display and a keyboard, a printer, and a Chinese information processing system. The instrument host is equipped with a 20-well reagent or antibody turntable and a 40-well specimen turntable. The system is controlled by a microcomputer and is fully automated. It can measure 20 immune specific protein items on 40 samples at a time.
The use of protein analyzers to detect plasma proteins has very important clinical significance. In the diagnosis of certain chronic diseases, plasma protein detection is still the earliest diagnostic indicator, such as chronic kidney disease, diabetes, and rheumatic diseases. The protein analyzer can be used to detect rheumatoid disease, diabetes, immune function, kidney function, cardiovascular disease, liver disease, blood disease, infection detection, nutritional status monitoring, etc.