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Human 25-Hydroxyvitamin D (25'-VD) Test

The 25-hydroxyvitamin-d test kit is used to determine the content of 25-hydroxy-Vitamin D (25-OH-VD) in human serum, it is mainly used for the auxiliary diagnosis of vitamin D deficiency-related diseases.

Testing Principle of Human 25-Hydroxyvitamin D (25'-VD) Test

  • The 25-hydroxy-Vitamin D in the sample and the monoclonal antibody in reagent 1 become immune complexes.

  • The antigenic determinant-DNA coupling template in Reagent 2 binds to the remaining monoclonal antibodies, and the unbound antigenic determinant-DNA coupling template and dNTPs are used to synthesize double-stranded DNA products under the action of polymerase.

  • This product binds to fluorescent dyes and will produce fluorescence which is proportional to the intensity of fluorescence and samples of 25-OH-VD levels.

  • Using a specific protein analyzer to measure the intensity of fluorescence, the concentration of 25-OH-VD is determined by comparing the fluorescence intensity of samples to the standard concentration.

Basic Parameters of Human 25-Hydroxyvitamin D (25'-VD) Test

  • Specimen: serum.

  • Test time: 350 seconds.

  • Packing: 25 Tests/ Kit.

  • Box Size: 17*10*8cm.

  • Storage: 2-8℃.

  • Shelf life: 1 year.

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