The testing reagent method has the characteristics of high sensitivity, strong specificity, and rapidity. It is currently widely used in the detection of viruses, agricultural and veterinary drugs, and mycotoxins. However, there are many steps in this method from sample addition, plate washing, color development to reading, and poor control of any of these steps may affect the accuracy of the test results. Therefore, the following points must be paid attention to in the actual measurement operation.
Pay attention to the temperature of the testing reagent, the temperature of the laboratory, and the temperature of the incubation when using the kit for testing. The refrigeration temperature of most kits is 2°C-8°C. Therefore, after taking it out of the refrigerator, do not rush to open the kit. Wait until the temperature of the kit reaches room temperature and there is no condensation on the surface of the kit before proceeding with the test; The reaction time of most kits is short after sample loading, and the ambient temperature will have a great influence on the test results. Therefore, controlling the ambient temperature is an important factor to ensure the test results of the kits; ensure that the temperature is uniform during incubation to prevent volatilization from drying out.
After the testing reagent returns to room temperature, take out the wells of the ELISA plate to be used, and put the unused ELISA plate wells back into the aluminum foil bag in time, add desiccant, seal tightly, and store at 2℃-8℃ in the refrigerator. Never put the microtiter plate that is not fully equilibrated to room temperature back into the aluminum foil bag, because at this time, due to the temperature difference, there will be moisture on the microtiter plate, which is not conducive to stable storage.
When using the testing reagent, you need to conduct a pre-experiment. Through the pre-experiment, you can familiarize yourself with the entire experimental process and find problems that may be overlooked; you can test whether the sample and the kit match. Once there is a mismatch, you will not waste too many samples; The experiment can also make a rough reference to the concentration of the target analyte in the sample to determine the appropriate dilution.